Plasmid

Part:BBa_K4161024

Designed by: Yunyi Ru   Group: iGEM22_DKU   (2022-10-10)


pET30a

This vector is used for plasmid construction and recombinant protein expression in E. coli BL21 DE3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 384
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 192
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 192
    Illegal BglII site found at 241
    Illegal BamHI site found at 198
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 384
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 384
    Illegal NgoMIV site found at 486
    Illegal NgoMIV site found at 2074
    Illegal NgoMIV site found at 2234
    Illegal NgoMIV site found at 5281
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3154


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Categories
Parameters
None